解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::While many antimicrobial peptides (AMPs) disrupt bacterial membranes, some translocate into bacteria and interfere with intracellular processes. Buforin II and DesHDAP1 are thought to kill bacteria by interacting with nucleic acids. Here, molecular modeling and experimental measurements are used to show that neither n...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12574
更新日期:2017-03-01 00:00:00
abstract::Jeotgalicoccus sp. 8456 OleTJE (CYP152L1) is a fatty acid decarboxylase cytochrome P450 that uses hydrogen peroxide (H2 O2 ) to catalyse production of terminal alkenes, which are industrially important chemicals with biofuel applications. We report enzyme fusion systems in which Streptomyces coelicolor alditol oxidase...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12581
更新日期:2017-03-01 00:00:00
abstract::ADP-forming acetyl-CoA synthetase (ACD) catalyzes the interconversion of acetyl-CoA and acetate. The related succinyl-CoA synthetase follows a three-step mechanism involving a single phosphoenzyme, but a novel four-step mechanism with two phosphoenzyme intermediates was proposed for Pyrococcus ACD. Characterization of...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.12573
更新日期:2017-02-01 00:00:00
abstract::LipL and Cpr19 are nonheme, mononuclear Fe(II)-dependent, α-ketoglutarate (αKG):UMP oxygenases that catalyze the formation of CO2 , succinate, phosphate, and uridine-5'-aldehyde, the last of which is a biosynthetic precursor for several nucleoside antibiotics that inhibit bacterial translocase I (MraY). To better unde...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12554
更新日期:2017-02-01 00:00:00
abstract::Recent studies have revealed that the sensory nervous system is involved in bone metabolism. However, the mechanism of communication between neurons and osteoblasts is yet to be elucidated. In this study, we investigated the signaling pathways between sensory neurons of the dorsal root ganglion (DRG) and the osteoblas...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12561
更新日期:2017-02-01 00:00:00
abstract::Mg2+ -dependent catechol-O-methyltransferases occur in animals as well as in bacteria, fungi and plants, often with a pronounced selectivity towards one of the substrate's hydroxyl groups. Here, we show that the bacterial MxSafC exhibits excellent regioselectivity for para as well as for meta methylation, depending on...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12530
更新日期:2017-01-01 00:00:00
abstract::Stability of green fluorescent protein (GFP) is sometimes important for a proper practical application of this protein. Random mutagenesis and targeted mutagenesis have been used to create better-folded variants of GFP, including recently reported extra-superfolder GFP. Our aim was to determine the crystal structure o...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12534
更新日期:2017-01-01 00:00:00
abstract::The ubiquitin-like modifier, FAT10, is involved in proteasomal degradation and antigen processing. As ubiquitin and the ubiquitin-like modifier, ISG15, cotranslationally modify proteins, we investigated whether FAT10 could also be conjugated to newly synthesized proteins. Indeed, we found that nascent proteins are mod...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12512
更新日期:2017-01-01 00:00:00
abstract::F-box proteins play pivotal roles in multiple cellular processes; however, little is known about their functions in glioma progression. In this study, we found that expression of the F-box and leucine-rich repeat protein 18 (FBXL18) is significantly upregulated in glioma tissues. Depletion of FBXL18 in glioma cells su...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12521
更新日期:2017-01-01 00:00:00
abstract::Fibroblast growth factor 11 (FGF11) is an intracellular FGF. Although induction of FGF11 by hypoxia has been observed in several cell types, the molecular function of FGF11 is not clearly understood yet. Here, we investigated the role of FGF11 under hypoxia. We identified hypoxia-inducible factor-1α (HIF-1α) as an int...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12547
更新日期:2017-01-01 00:00:00
abstract::The UDP-GlcNAc:lysosomal enzyme, N-acetylglucosamine-1-phosphotransferase (GlcNAc-1-PT), is an α2 β2 γ2 hexamer that mediates the initial step in the formation of the mannose 6-phosphate targeting signal on newly synthesized lysosomal acid hydrolases. The GNPTAB gene encodes the 1256 amino acid long α/β precursor whic...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12525
更新日期:2017-01-01 00:00:00
abstract::Activation of AMP-activated protein kinase (AMPK) has been viewed as an important target for the treatment of insulin resistance. Here, by proteomic analysis, we found that expression of heat shock protein beta-1 (HSPB1) was induced by the AMP analog 5-aminoimidazole-4-carboxamide 1-β-D-ribofuranoside in palmitate-ind...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12516
更新日期:2017-01-01 00:00:00
abstract::The Bacillus thuringiensis Cry toxin causes swelling and necrosis in insect cells, but the route(s) and significance of the water influx involved in its cytotoxicity are unclear. Here, we assessed the role of aquaporins (AQPs), known as water channels, in Cry toxin intoxication. An AQP inhibitor did not interfere with...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12506
更新日期:2017-01-01 00:00:00
abstract::The human pathogen Vibrio vulnificus undergoes phase variation among colonial morphotypes, including a virulent opaque form which produces capsular polysaccharide (CPS) and a translucent phenotype that produces little or no CPS and is attenuated. Here, we found that a V. vulnificus mutant defective for RfaH antitermin...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12490
更新日期:2016-12-01 00:00:00
abstract::We have developed a high-throughput sequencing approach that enables us to determine terminal telomere sequences from tens of thousands of individual Schizosaccharomyces pombe telomeres. This method provides unprecedented coverage of telomeric sequence complexity in fission yeast. S. pombe telomeres are composed of mo...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12444
更新日期:2016-12-01 00:00:00
abstract::Development of targeted therapies for triple-negative breast cancer (TNBC, a more aggressive subtype) is an unmet medical need. We analyzed data from 887 patients with invasive breast cancer and observed that increased Wnt and histone deacetylase (HDAC) activities are associated with estrogen receptor 1 (ESR1) and pro...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12496
更新日期:2016-12-01 00:00:00
abstract::Glycogen metabolism in β-cells may affect downstream metabolic pathways controlling insulin release. We examined glycogen metabolism in human islets and in the rodent-derived INS-1 832/13 β-cells and found them to express the same isoforms of key enzymes required for glycogen metabolism. Our findings indicate that gly...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12460
更新日期:2016-12-01 00:00:00
abstract::Streptococcus pneumoniae (pneumococcus) has evolved sophisticated strategies to survive in several niches within the human body either as a harmless commensal or as a serious pathogen causing a variety of diseases. The dynamic interaction between pneumococci and resident host cells during colonization of the upper res...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.12352
更新日期:2016-11-01 00:00:00
abstract::The intestinal microbiota is essential for nutrient acquisition, immune development, and exclusion of invading pathogens. The upper respiratory tract (URT) microbiota is less well studied and does not appear to abide by many of the paradigms of the gastrointestinal tract. Decades of carriage studies in children have d...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.12455
更新日期:2016-11-01 00:00:00
abstract::Regulation of integrin affinity for its ligand is essential for cell adhesion and migration. Here, we found that direct interaction of vimentin with integrin β1 can enhance binding of integrin α5β1 to its ligand, fibronectin. Conversely, blocking the interaction reduced fibronectin binding, cell migration on a fibrone...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12430
更新日期:2016-10-01 00:00:00
abstract::VP64 is widely used as a transcriptional activator to investigate the biological function of genes, but its potential for application in genetic improvement of crops has not been fully investigated. Here, we characterized an OsMYB1R1-VP64 fusion protein that enhanced the grain yield of rice cultivar 'Kita-ake' by 35%....
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12374
更新日期:2016-10-01 00:00:00
abstract::Mitochondria continually fuse and divide to dynamically adapt to changes in metabolism and stress. Mitochondrial dynamics are also required for mitochondrial DNA (mtDNA) integrity; however, the underlying reason is not known. In this study, we examined the link between mitochondrial fusion and mtDNA maintenance in Sch...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12432
更新日期:2016-10-01 00:00:00
abstract::Glycosaminoglycans are known to be associated with extracellular amyloid deposits of various amyloidogenic proteins. In this study, we found that the glycosaminoglycan heparin greatly accelerates the elongation step in fibril formation by the N-terminal 1-83 fragment of human apolipoprotein A-I (apoA-I), especially in...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12426
更新日期:2016-10-01 00:00:00
abstract::An interpretation of alternative crystal structures of the erythropoietin receptor, with and without ligand, led to the proposal of a scissor-like mechanism of activation. This model has been propagated in the literature and is still being used to interpret crystal structures of related type-I cytokine receptors. Here...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.12340
更新日期:2016-09-01 00:00:00
abstract::Neoplastic cells metabolize higher amounts of glucose relative to normal cells in order to cover increased energetic and anabolic needs. Inhibition of the glycolytic enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3) diminishes cancer cell proliferation and tumour growth in animals. In this work, w...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12338
更新日期:2016-09-01 00:00:00
abstract::MeCP2 is a chromatin-associated protein that is mutated in Rett syndrome. Its methyl-CpG-binding domain interacts with DNA containing methylated cytosine, but other modes of recruitment to the genome have also been proposed. Here, we use in vitro and in vivo assays to investigate the DNA binding specificity of two AT-...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12328
更新日期:2016-09-01 00:00:00
abstract::While the majority of studies have focused on the biological roles of heparin-binding proteins, relatively little is known about their key residues and structural elements responsible for heparin interaction. In this study, we employed the IgG-binding domain B1 of Streptococcal protein G as a miniature scaffold to inv...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12361
更新日期:2016-09-01 00:00:00
abstract::We solved the crystal structure of a functionally uncharacterized protein, HP0902, from Helicobacter pylori. Its structure demonstrated an all-β cupin fold that cannot bind metal ions due to the absence of a metal-binding histidine that is conserved in many metallo-cupins. In contrast, isothermal titration calorimetry...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12332
更新日期:2016-09-01 00:00:00
abstract::γ-Glutamyl kinase (GK; the PRO1 gene product) is a key enzyme in the Saccharomyces cerevisiae proline biosynthesis pathway. Δpro1 cells are more sensitive to various stresses than wild-type cells, suggesting that GK has an alternative function independent of proline biosynthesis. We show that PRO1 genetically interact...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12318
更新日期:2016-09-01 00:00:00
abstract::The enzyme glucose-6-phosphate dehydrogenase from Trypanosoma cruzi (TcG6PDH) catalyses the first step of the pentose phosphate pathway (PPP) and is considered a promising target for the discovery of a new drug against Chagas diseases. In the present work, we describe the crystal structure of TcG6PDH obtained in a ter...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12276
更新日期:2016-08-01 00:00:00
abstract::Astrocyte-elevated gene-1 (AEG-1) positively regulates tumor progression and metastasis. Here, we document that AEG-1 upregulates transcription of the membrane protein tetraspanin 8 (TSPAN8). Knocking down TSPAN8 in AEG-1-overexpressing human hepatocellular carcinoma (HCC) cells markedly inhibited invasion and migrati...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12268
更新日期:2016-08-01 00:00:00
abstract::Cellular retinoic acid binding proteins (CRABPs) bind all-trans-retinoic acid (atRA) tightly. This study aimed to determine whether atRA is channeled directly to cytochrome P450 (CYP) CYP26B1 by CRABPs, and whether CRABPs interact directly with CYP26B1. atRA bound to CRABPs (holo-CRABP) was efficiently metabolized by ...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12303
更新日期:2016-08-01 00:00:00
abstract::Long noncoding RNA (lncRNA) are emerging as important regulators of diverse biological functions. Although mechanistic models are starting to emerge, it is also clear that the lncRNA field needs appropriate model systems in order to better elucidate the functions of lncRNA and their roles in both physiological and pat...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.12308
更新日期:2016-08-01 00:00:00
abstract::The efficiency of visible light in inducing fluorescence quenching in the isolated light-harvesting complex II (LHCII) of higher plants is investigated by action spectroscopy in the visible portion of photosynthetic active radiation. The efficiency spectrum displays a relatively homogenous quenching yield across the m...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12279
更新日期:2016-08-01 00:00:00
abstract::Syndecans are transmembrane heparan sulfate proteoglycans, with roles in development, tumorigenesis and inflammation, and growing evidence for involvement in tissue regeneration. This is a fast developing field with the prospect of utilizing tissue engineering and biomaterials in novel therapies. Syndecan receptors ar...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.12280
更新日期:2016-08-01 00:00:00
abstract::MicroRNA (miRNA) play a pivotal role in the development of liver fibrosis. However, the functions of miRNA in hepatitis C virus (HCV)-related liver fibrosis remain unclear. In this study, we systematically analyzed the microarray data of the serum miRNA in patients with HCV-induced hepatic fibrosis. Among 41 dysregula...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12309
更新日期:2016-08-01 00:00:00
abstract::C2cd4c, encoded by a gene belonging to the C2cd4 family, contains a C2 domain conserved across species and is localized to the cytoplasm. To examine the role of C2cd4c in the pancreas, we studied its localization and generated C2cd4c knockout (KO) mice. C2cd4c was expressed in pancreatic endocrine progenitors at early...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12271
更新日期:2016-08-01 00:00:00
abstract::Intravital multiphoton imaging allows visualization of infections and pathogenic mechanisms within intact organs in their physiological context. Today, most organs of mice and rats are applicable to in vivo or ex vivo imaging, opening completely new avenues for many researchers. Advances in fluorescent labeling of pat...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.12122
更新日期:2016-07-01 00:00:00
abstract::We previously showed that P4-ATPases, ATP10A/ATP8B1, and ATP11A/ATP11C have flippase activities toward phosphatidylcholine (PC), and aminophospholipids [phosphatidylserine (PS) and phosphatidylethanolamine], respectively. Here, we investigate the effect of PC-specific flippases versus aminophospholipid-specific flippa...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12247
更新日期:2016-07-01 00:00:00
abstract::In the first committed reaction of chlorophyll biosynthesis, magnesium chelatase couples ATP hydrolysis to the thermodynamically unfavorable Mg(2+) insertion into protoporphyrin IX (ΔG°' of circa 25-33 kJ·mol(-1) ). We explored the thermodynamic constraints on magnesium chelatase and demonstrate the effect of nucleoti...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12214
更新日期:2016-06-01 00:00:00